GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE224686 Query DataSets for GSE224686
Status Public on Feb 15, 2024
Title Divergent learning-related transcriptional states of cortical glutamatergic neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Experience-dependent gene expression reshapes neural circuits, permitting learning of knowledge and skills. However, the diversity of plasticity-related transcriptional responses across neurons underlying learning remains poorly understood. Here, we analyzed single-nucleus transcriptomes of L2/3 glutamatergic neurons of the primary motor cortex after motor skill training or home-cage control in water-restricted mice.
Overall design The caudal forelimb area of primary motor cortex for 6 mice was analyzed using snRNAseq (shallow and deep sequencing was performed for each mouse). 3 mice were control, and 3 were trained on a lever-press task.
barcode suffixes (-1 to -6) correspond to the samples titled "M1 nuclei, control, mouse1, shallow snRNAseq ", "...mouse2...", "…mouse3…", etc., respectively
Contributor(s) Dunton KL, Hedrick NG, Meamardoost S, Ren C, Howe JR, Wang J, Root CM, Gunawan R, Komiyama T, Zhang Y, Hwang EJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 06, 2023
Last update date Feb 15, 2024
Contact name Ying Zhang
Organization name University of Rhode Island
Department Cell and Molecular Biology
Street address 120 Flagg Rd
City Kingston
State/province RI
ZIP/Postal code 02881-2015
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7029483 M1 nuclei, control, mouse1, shallow snRNAseq
GSM7029484 M1 nuclei, control, mouse1, deep snRNAseq
GSM7029485 M1 nuclei, train, mouse2, shallow snRNAseq
BioProject PRJNA932053

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224686_barcodes.tsv.gz 259.1 Kb (ftp)(http) TSV
GSE224686_features.tsv.gz 272.8 Kb (ftp)(http) TSV
GSE224686_matrix.mtx.gz 462.0 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap