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| Status |
Public on Feb 03, 2024 |
| Title |
Chromatin landscape of budding yeast acquiring H3K9 methylation and its reader molecule HP1 [ChIP-Seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Histone H3 lysine 9 (H3K9) methylation and heterochromatin protein 1 (HP1) are well conserved epigenetic silencing mark and its reader molecule, and crucial for heterochromatin formation. However, the details of the importance of H3K9 methylation and HP1 in heterochromatin formation still remain unclear. One of the reason is the redundancy problem, as there are multiple reader molecules for H3K9 methylation, including HP1, and HP1 itself functions as a hub that recruits various effector molecules. To overcome the redundancy issue, we took synthetic biology approach and introduced H3K9 methylation and HP1 into budding yeast Saccharomyces cerevisiae, which does not have this system, and examined its impact on transcription and chromatin compaction. We observed that mammalian H3K9 methyltransferase can induce genome wide H3K9 di- and tri-methylation (H3K9me2,3) in budding yeast, and that HP1 accumulates over the H3K9 methylated regions. However, H3K9 methylation occurred mainly in the gene body region of the genes and excluded around TSS where H3K9ac pre-exists. Correspondingly, expression of H3K9 methyltransferase and HP1 did not affect transcription in budding yeast, including repression. ATAC-seq analysis also showed no impact on chromatin accessibility, and Hi-C-seq analysis of chromatin 3D structure revealed no significant differences. These results suggest that even though H3K9 methylation and recruitment of HP1 play essential roles in epigenetic regulation of heterochromatin, they are not sufficient to build up heterochromatin, at least at gene body regions, and further participation of effector molecules, including downstream factors of HP1, is required.
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| Overall design |
mSUV39H1 and HP1 were expressed in S. Cerevisiae, and the H3K9 methylated regions and the localization of HP1 were analyzed by ChIP-seq.
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| Contributor(s) |
Shimizu T, Fukuda K, Nakayama J, Oki M, Shinkai Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 06, 2023 |
| Last update date |
Feb 03, 2024 |
| Contact name |
Taiki Shimizu |
| E-mail(s) |
taiki.shimizu@riken.jp
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| Organization name |
RIKEN
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| Street address |
2-1,Hirosawa,Wako
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| City |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
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| Platforms (1) |
| GPL26171 |
HiSeq X Ten (Saccharomyces cerevisiae) |
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| Samples (18)
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| GSM7028195 |
mock/HP1β strain, rep1, H3K9me2 |
| GSM7028196 |
SUV39H1/mock strain, rep1, H3K9me2 |
| GSM7028197 |
SUV39H1/mock strain, rep2, H3K9me2 |
| GSM7028198 |
SUV39H1/HP1β strain, rep1, HP1β |
| GSM7028199 |
SUV39H1/HP1β strain, rep2, HP1β |
| GSM7028200 |
SUV39H1/HP1β strain, rep1, H3K9me2 |
| GSM7028201 |
SUV39H1/HP1β strain, rep2, H3K9me2 |
| GSM7028202 |
SUV39H1/HP1β strain, rep1, H3K9me3 |
| GSM7028203 |
SUV39H1/HP1β strain, rep2, H3K9me3 |
| GSM7028204 |
mock/HP1β strain, rep1, input |
| GSM7028205 |
mock/HP1β strain, rep2, input |
| GSM7028206 |
SUV39H1/mock strain, rep1, input |
| GSM7028207 |
SUV39H1/mock strain, rep2, input |
| GSM7028208 |
SUV39H1/HP1β strain, rep1, input |
| GSM7028209 |
SUV39H1/HP1β strain, rep2, input |
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| This SubSeries is part of SuperSeries: |
| GSE224641 |
Chromatin landscape of budding yeast acquiring H3K9 methylation and its reader molecule HP1 |
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| Relations |
| BioProject |
PRJNA934496 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224639_RAW.tar |
19.9 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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