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Series GSE224354 Query DataSets for GSE224354
Status Public on Jul 05, 2024
Title Alveolar epithelial cells mitigate neutrophilic inflammation in acute lung injury through regulating mitochondrial fatty acid oxidation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Carnitine palmitoyltransferase 1a (CPT1a) is the key regulator of mitochondrial long-chain fatty acid beta-oxidation (LCFAO). However, the functional significance of AT2 cell-specific LCFAO at baseline and during acute lung injury (ALI) is not fully understood. In this study, Murine models of AT2 cell-specific Cpt1a deletion were generated for investigating the role of CPT1a in regulating AT2 cell function and the severity of lipopolysaccharide-induced murine ALI models.
 
Overall design AT2 cells were isolated from the lungs from mice with AT2 cell-specific Cpt1a deletion (Cpt1a-KO) and the control (Sftpc-CreERt2 +/-) at baseline and 24 hours after intra-tracheal LPS administration. To investigate whether impaired LCFAO in AT2 cells regulates the responses of alveolar macrophages in ALI, we also isolated alveolar macrophages from Cpt1a-KO and control mice at 4 hours after intra-tracheal LPS administration, and alveolar macrophages from control mice at baseline. Alveolar macrophages from 2 mice were pooled to make one sample for trancriptomic analysis.
 
Contributor(s) Chung K, Hsu C, Yu C
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Submission date Feb 02, 2023
Last update date Jul 05, 2024
Contact name Kuei-Pin Chung
Organization name National Taiwan University Hospital
Department Laboratory Medicine
Street address No 7, Chung-Shan South Rd
City Taipei
ZIP/Postal code 100
Country Taiwan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (34)
GSM7020369 AT2 cells from control mouse (#740), baseline
GSM7020370 AT2 cells from control mouse (#741), baseline
GSM7020371 AT2 cells from control mouse (#742), baseline
Relations
BioProject PRJNA930707

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224354_read_count_AT2.txt.gz 904.5 Kb (ftp)(http) TXT
GSE224354_read_count_Macrophage.txt.gz 972.1 Kb (ftp)(http) TXT
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