|
| Status |
Public on Oct 02, 2023 |
| Title |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Pancreatic ductal adenocarcinoma (PDAC) tumours carry multiple gene mutations and respond poorly to treatments. There is currently an unmet need for drug carriers that can deliver multiple gene cargoes to high solid tumour burden like PDAC. Here, we report a dual-targeted extracellular vesicle (EV) carrying high loads of RNA that effectively suppresses large PDAC tumours in mice. The EV surface contains a CD64 protein that has a tissue targeting peptide and a humanized monoclonal antibody. Cells sequentially transfected with plasmid DNAs encoding for the RNA and protein of interest by Transwell®-based asymmetric cell electroporation released abundant targeted EVs with high RNA loading. Together with a low dose chemotherapy drug, Gemcitabine, dual-targeted EVs effectively suppressed large orthotopic PANC-1 and patient derived xenograft tumours in mice and extended animal survival. This work presents a clinically accessible and scalable way to produce abundant EVs for delivering multiple gene cargoes to large solid tumours.
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| |
|
| Overall design |
MOUSE_arrays
|
| |
|
| Contributor(s) |
Wang K, Lee I, Fallen S, Lee L, Chiang N |
| Citation(s) |
37872156 |
| |
| Submission date |
Jan 20, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Inyoul Lee |
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Ave. North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (14)
|
| GSM6947333 |
Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep3 |
| GSM6947334 |
Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep1 |
| GSM6947335 |
Gene expression of PBS treated MEF-derived EV_rep2 |
| GSM6947336 |
Gene expression of Control MEF-derived EV_rep2 |
| GSM6947337 |
Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep2 |
| GSM6947338 |
Gene expression of PBS treated MEF-derived EV_rep3 |
| GSM6947339 |
Gene expression of PBS treated MEF-derived EV_rep1 |
| GSM6947340 |
Gene expression of Control MEF-derived EV_rep1 |
| GSM6947341 |
Gene expression of PBS treated MEF-derived EV_rep4 |
| GSM6947342 |
Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV_rep1 |
| GSM6947343 |
Gene expression of PBS treated MEF-derived EV_rep5 |
| GSM6947344 |
Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV_rep2 |
| GSM6947345 |
Gene expression of Control MEF-derived EV_rep3 |
| GSM6947346 |
Gene expression of Control MEF-derived EV_rep4 |
|
| This SubSeries is part of SuperSeries: |
| GSE223409 |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer |
|
| Relations |
| BioProject |
PRJNA925942 |
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