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Series GSE223268 Query DataSets for GSE223268
Status Public on Jan 14, 2025
Title Eosinophils Restrain Humoral Alloimmunity after Lung Transplantation [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary While the function of many leukocytes in transplant biology has been well defined, the role of eosinophils is controversial and remains poorly explored. Conflicting data exist regarding eosinophils’ role in alloimmunity based on context and organ type. Due to their prevalence in the lung, and their defined role in other pulmonary pathology such as asthma, we set out to explore the role of eosinophils in the long-term maintenance of the lung allograft. We noted that depletion of eosinophils results in the generation of donor-specific antibodies. Eosinophil depletion increased memory B cell, plasma cell, and antibody secreting cell differentiation and resulted in de novo-generation of follicular germinal centers. Germinal center formation depended on the expansion of CD4+Foxp3-Bcl6+CXCR5+PD1+ T follicular helper cells (Tfh) which increase in number after eosinophil depletion. Mechanistically, we demonstrate that eosinophils prevent Tfh generation by acting as the dominant source of IFN-γ in an established lung allograft, thus facilitating Th1 rather than Tfh polarization of naïve CD4+ T cells. Our data thus describe a unique and previously unknown role for eosinophils in maintaining allograft tolerance and suggests that indiscriminate administration of eosinophil-lytic corticosteroids for treatment of acute cellular rejection may inadvertently promote humoral alloimmunity.
 
Overall design Lung allograft samples harvested and digested to single cells. zmlx175 and zmlx176 were eosinophil deficient group while zmlx180 and zmlx181 were eosinophil sufficent group as control. No replicates for any sample.
 
Contributor(s) Tallon L, Courneya J, Krupnick A, Mei Z
Citation(s) 38329123
Submission date Jan 19, 2023
Last update date Jan 24, 2025
Contact name JP Courneya
Organization name University of Maryland, Baltimore
Street address 685 W Baltimore St., HSF2 Room 480
City Baltimore
State/province United States of America
ZIP/Postal code 21201
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM6944101 zmlx175_Eosinophil deficient
GSM6944102 zmlx176_Eosinophil deficient
GSM6944103 zmlx180_Eosinophil deficient
This SubSeries is part of SuperSeries:
GSE223907 Eosinophils Restrain Humoral Alloimmunity after Lung Transplantation
Relations
BioProject PRJNA925481

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Supplementary file Size Download File type/resource
GSE223268_RAW.tar 243.3 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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