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Series GSE223077 Query DataSets for GSE223077
Status Public on Sep 20, 2023
Title Bulk RNA-seq of CRISPRi ADGRG6-kd iAT2 following cigarette smoke exposure
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed bulk transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of ADGRG6. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Cells were plated at an air-liquid interface, then subsequently exposed to air or 5% cigarette smoke using a VitroCell smoke robot. Cells were harvested for bulk RNA sequencing 8 hours post cigarette smoke exposure
Overall design Bulk transcriptomic analysis of iPSC-derived type 2 alveolar epithelial cells (iAT2) comparing wild-type cells with CRISPRi-mediated knockdown of ADGRG6 following exposure to air or 5% cigarette smoke exposure
Contributor(s) Werder RB, Wilson A, Bawa P
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Submission date Jan 17, 2023
Last update date Sep 20, 2023
Contact name Pushpinder Bawa
Organization name Center for Regenerative Medicine
Department Boston University Medical Campus
Street address 670 Albany St
City Boston
State/province Massachusetts
ZIP/Postal code 02118
Country USA
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (12)
GSM6939358 WA_WR_0726_1
GSM6939359 WA_WR_0726_2
GSM6939360 WA_WR_0726_3
BioProject PRJNA924805

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