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| Status |
Public on Apr 28, 2023 |
| Title |
ChIP-seq of in vitro differentiated naïve and Th9 cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate changes in chromatin accessibility over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production
We then performed Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K4me1, K3K4me3, and H3K27Ac in cells from 2 different human donors and from 2 mice at 4 time points.
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| Overall design |
Peaks for various histone marks normalized to input
|
| |
|
| Contributor(s) |
Schwartz DM, Son A |
| Citation(s) |
37106040 |
| |
| Submission date |
Jan 14, 2023 |
| Last update date |
Apr 29, 2023 |
| Contact name |
Daniella Schwartz |
| E-mail(s) |
Daniella.Schwartz@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Street address |
200 Lothrop St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15216 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (64)
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| This SubSeries is part of SuperSeries: |
| GSE222910 |
ATAC-seq, ChIP-seq and RNA-seq of in vitro differentiated naïve, Th1, and Th9 cells |
|
| Relations |
| BioProject |
PRJNA923820 |
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