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Series GSE222860 Query DataSets for GSE222860
Status Public on Apr 18, 2024
Title Plasmodium yoelii gender-specific gametocyte transcriptome
Organism Plasmodium yoelii
Experiment type Expression profiling by high throughput sequencing
Summary In order to identify RNA binding proteins that are essential for male gametogenesis in the rodent malaria parasite P. yoelii, we conducted RNA-seq analysis to identify RBPs that are specifically expressed in male gametocytes.
 
Overall design A double fluorescence reporter strain DFsc7, which allowed for specific expression of GFP in male gametocytes and mCherry in female gametocytes, was utilized to induce gametocytes in mice. Subsequently, the male and female gametocytes were sorted using fluorescence-activated cell sorting (FACS), and RNA-seq analysis was conducted to generate gender-specific transcriptomes. Addition: Through Principal Component Analysis (PCA) analysis, the initial sample, Male biol rep1, showed poor reproducibility in RNA-seq data. Subsequently, we re-sorted and obtained three samples of male gametes (Py17XNL_MG_Rep1, Py17XNL_MG_Rep2, and Py17XNL_MG_Rep3) for RNA-seq, which yielded more reliable data. The corresponding Supplementary files for the improved data are edgeR_male_vs_female.csv.gz and male_vs_female_TPM.csv.gz.
 
Contributor(s) Mo X, Li Z, Guan J
Citation(s) 38824128
Submission date Jan 13, 2023
Last update date Jun 26, 2024
Contact name jiepeng guan
E-mail(s) guanjiepengn@gmail.com
Organization name Xiamen University
Street address Xiamen University
City Xiamen
ZIP/Postal code 361105
Country China
 
Platforms (1)
GPL29225 Illumina NovaSeq 6000 (Plasmodium yoelii)
Samples (9)
GSM6934331 Male biol rep1
GSM6934332 Male biol rep2
GSM6934333 Male biol rep3
Relations
BioProject PRJNA923669

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Supplementary file Size Download File type/resource
GSE222860_Female.txt.gz 903.1 Kb (ftp)(http) TXT
GSE222860_Male.txt.gz 336.3 Kb (ftp)(http) TXT
GSE222860_edgeR_male_vs_female.csv.gz 348.7 Kb (ftp)(http) CSV
GSE222860_male_vs_female_TPM.csv.gz 392.5 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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