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| Status |
Public on Jan 12, 2023 |
| Title |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness [CTD] |
| Platform organisms |
Escherichia coli; Triticum aestivum; Spodoptera frugiperda; Homo sapiens; Sus scrofa; Bos taurus; Capra hircus |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
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| Summary |
We used protein arrays to measure IgG autoantibodies associated with Connective Tissue Diseases (CTDs) and Anti-Cytokine Antibodies (ACA) in patients with non-COVID-19 infections such as influenza
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| Overall design |
Protein arrays were constructed by coupling antigens to carboxylated magnetic beads. Arrays consisting of conjugated beads were then used to probe patient serum or plasma samples, healthy control samples, and autoimmune prototype samples. Samples were run in duplicate except one sample (UP67 D7) which was run in quadruplicate. for A Luminex FLEXMAP 3D® system was used to quantitatively measure autoantibody reactivity in each sample in units of mean flourescent intensity (MFI). Positive antibody hits were statistically identified by a 5 standard deviation cutoff calculated from the MFI of healthy controls.
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| Contributor(s) |
Feng A, Yang EY, Moore AR, Dhingra S, Chang SE, Yin X, Pi R, Mack E, Völkel S, Geßner R, Gündisch M, Neubauer A, Renz H, Tsiodras S, Fragkou PC, Asuni A, Levitt JE, Wilson JG, Leong MW, Lumb JH, Mao R, Pinedo K, Roque JA, Richards CM, Stabile M, Swaminathan G, Salagianni M, Triantafyllia V, Bertrams W, Blish CA, Carette JE, Frankovich J, Meffre E, Nadeau K, Singh U, Wang TT, Luning Prak ET, Herold S, Andreakos E, Schmeck B, Skevaki C, Rogers AJ, Utz P |
| Citation(s) |
36752204 |
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| Submission date |
Jan 12, 2023 |
| Last update date |
Apr 15, 2023 |
| Contact name |
Allan Feng |
| E-mail(s) |
afeng1@stanford.edu
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| Phone |
2055678956
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| Organization name |
Stanford School of Medicine
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| Department |
Imm/Rheum
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| Lab |
Utz
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| Street address |
269 Campus Dr
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (355)
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| This SubSeries is part of SuperSeries: |
| GSE222765 |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness |
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| Relations |
| BioProject |
PRJNA923336 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222764_CTD_Array_Platform_Raw_MFI.xlsx |
273.4 Kb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
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