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| Status |
Public on Feb 17, 2023 |
| Title |
Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [CUT&RUN] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
The loss of discrete morphological traits, the most common evolutionary transition, is typically driven by changes in expression of developmental genes. Mutations accumulating in regulatory elements of these genes can disrupt DNA binding sites for transcription factors patterning their spatial expression, or delete entire enhancers. Regulatory elements, however, may in principle be silenced through other mechanisms, including changes in chromatin accessibility, or the emergence of repressive elements. Here, we show that an increase in chromatin accessibility at the pigmentation gene yellow, combined with the gain of a repressor site, underlie the evolutionary loss of a spot pigmentation pattern on the wings of a Drosophila species. The evolutionary gain of accessibility of this repressive element is regulated by E93, a transcription factor governing the progress of metamorphosis. This convoluted evolutionary scenario contrasts with the classical parsimonious mutational paths generally envisioned and often documented for morphological losses. It illustrates for the first time how evolutionary changes in chromatin accessibility may directly contribute to morphological diversification.
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| Overall design |
We profiled the signal enrichment of H3K27ac at the stage (67% of pupal development) when spot activity was repressed in D. melanogaster using CUT&RUN.
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| Contributor(s) |
Ling L, Gompel N |
| Citation(s) |
36800429 |
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| Submission date |
Jan 12, 2023 |
| Last update date |
May 19, 2023 |
| Contact name |
Nicolas Gompel |
| Organization name |
Ludwig Maximilian University of Munich
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| Department |
Faculty of Biology
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| Street address |
Grosshaderner Str. 2
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| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (1) |
| GPL19951 |
Illumina HiSeq 1500 (Drosophila melanogaster) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE222717 |
Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila. |
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| Relations |
| BioProject |
PRJNA923258 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222715_RAW.tar |
194.3 Mb |
(http)(custom) |
TAR (of BW, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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