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| Status |
Public on Jan 12, 2024 |
| Title |
Transcriptome profiling of mRNAs in cortex of rats with cerebral ischemia and reperfusion treated by acupuncture |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed transcriptomic characteristic of motor cortex from Sprague-Dawley rats brain with cerebral ischemia and reperfusion (CIR) treated by acupuncture.
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| Overall design |
We selected 3 Sprague-Dawley rats with CIR treated by electroacupuncture at GB20 (Fengchi acupoints). The motor cortex was stripped from brain tissue, and total RNAs were extracted according to the manufacturer’s instructions. Qualified total RNA was further purified. We complete the construction of sequencing sample library. The number of fragments of each gene was counted, and then normalized. Finally, the FPKM value of each gene was calculated by Perl script.
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| Contributor(s) |
Xu M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 12, 2023 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Ming-Shu Xu |
| Organization name |
Shanghai University of Traditional Chinese Medicine
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| Department |
Shanghai Research Institute of Acupuncture and Meridian
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| Street address |
No. 650 South Wanping Road, Xuhui District
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| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (3) |
| GSM6929215 |
cerebral ischemia and reperfusion with electroacupuncture group, No.12 |
| GSM6929216 |
cerebral ischemia and reperfusion with electroacupuncture group, No.24-4 |
| GSM6929217 |
cerebral ischemia and reperfusion with electroacupuncture group, No.33 |
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| Relations |
| BioProject |
PRJNA923245 |
