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Series GSE222699 Query DataSets for GSE222699
Status Public on May 24, 2024
Title The OTX2-regulated alternative splicing landscape controls differentiation and reconstitutes the developmental origins of Group 3 medulloblastoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing was performed on Group 3 medulloblastoma tumorspheres following PPHLN Morpholino treatment or scramble control.
Overall design A custom splice-blocking morpholino to induce exon skipping was designed for PPHLN1 (ENSG00000134283.13, exon 6, 5’-AGAGTCTAGCTCAAAACTCACCTCT-3’). A concentration of 1 µM in ddH20 was added to HDMB03 single cell suspensions cultured in ultra-low-attachment plates for 5 days prior to RNA extraction.
Contributor(s) Werbowetski-Ogilvie T
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Submission date Jan 12, 2023
Last update date May 24, 2024
Contact name Michael D Taylor
Organization name The Hospital for Sick Children
Department Development and Stem Cell Biology
Street address 686 Bay St
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A4
Country Canada
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM6929162 HDMB03_Ctrl_1
GSM6929163 HDMB03_Ctrl_2
GSM6929164 HDMB03_Ctrl_3
BioProject PRJNA923174

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE222699_RAW.tar 2.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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