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| Status |
Public on Apr 26, 2023 |
| Title |
Single nucleus RNAseq of midbrain dopaminergic neuronal nuclei isolated by FACS |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
While many studies have performed single cell profiling of dopamine (DA) neurons in mice, they have largely been limited by the number of DA neurons captured. Here, we performed single-nucleus RNAseq on DA neurons to refine the DA subtype landscape.
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| Overall design |
Two replicates from a total of n=5 DAT-Cre mice with a nuclear GFP reporter. Samples contain tissue from midbrains after isolation of DAT+ nuclei via FACS.
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| Contributor(s) |
Gaertner Z, Azcorra M, Awatramani R, Dombeck DA |
| Citation(s) |
37537242 |
| |
| Submission date |
Jan 10, 2023 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Zachary Gaertner |
| E-mail(s) |
zachary.gaertner@northwestern.edu
|
| Organization name |
Northwestern University, Feinberg School of Medicine
|
| Department |
Neurology
|
| Lab |
Awatramani Lab
|
| Street address |
320 E Superior, Searle 7-450
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA922521 |
