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Series GSE222105 Query DataSets for GSE222105
Status Public on May 15, 2023
Title Defining prepubertal primate spermatogonial stem cells to address fertility preservation in prepubertal boys
Organisms Macaca mulatta; Papio anubis
Experiment type Expression profiling by high throughput sequencing
Summary Antineoplastic treatments for cancer and other non-malignant disorders can result in male long-term or permanent infertility by ablating spermatogonial stem cells (SSCs). Preserving the future fertility of prepubertal patients faced with this prospect cannot rely on sperm banking. SSC transplantation using testicular tissue harvested before sterilizing treatment is a promising approach for restoring fertility, but lack of exclusive biomarkers which can unequivocally identify prepubertal SSCs in any primate species limits the therapeutic potential. To address this, we performed single-cell RNA-seq on unselected testis cells from immature baboons and macaques and compared these cells with published data from pre-pubertal human germ cells functionally-defined mouse SSCs. We found discrete groups of human spermatogonia while baboon and rhesus spermatogonia appeared less heterogenous. Cross-species analysis revealed cell types analogous to human SSCs in baboon and rhesus germ cells, but comparison with mouse SSCs revealed significant differences with primate SSCs. Indeed, a core component of a human SSC gene expression signature was conserved in baboon and rhesus spermatogonia and absent from mouse SSCs. These results further resolve the identity of pre-pubertal human SSCs and define novel pathways that could be leveraged for advancing their selection and propagation in vitro.
Overall design Single-cell transcriptomes of testicular cells from two immature baboons (aged 15 and 20 months) along with two rhesus monkeys (newborn and 26 months old) were analyzed.
Contributor(s) Anukriti S, Vargas S, Hermann BP
Citation(s) 36902187
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HD090007 Origin and functional significance of the spermatogonial stem cell transcriptome barcode THE UNIVERSITY OF TEXAS AT SAN ANTONIO Brian Peter Hermann
U01 DA054170 Advancing brain health research through male germline editing in marmosets THE UNIVERSITY OF TEXAS AT SAN ANTONIO Brian Peter Hermann
Submission date Jan 03, 2023
Last update date May 17, 2023
Contact name Brian P. Hermann
Phone 210-458-8047
Organization name University of Texas at San Antonio
Department Department of Biology
Street address 1 UTSA Circle
City San Antonio
State/province TX
ZIP/Postal code 78249
Country USA
Platforms (3)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
GPL27943 Illumina NovaSeq 6000 (Macaca mulatta)
GPL30489 Illumina NovaSeq 6000 (Papio anubis)
Samples (6)
GSM6914875 Baboon36506A
GSM6914876 Baboon36506B
GSM6914877 Baboon37979
BioProject PRJNA917768

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Supplementary file Size Download File type/resource
GSE222105_RAW.tar 386.1 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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