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Series GSE222064 Query DataSets for GSE222064
Status Public on Jan 04, 2023
Title A neural substrate for negative affect dictates female parental behaviour
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Parental behaviours are essential to secure survival and wellbeing of newborns. Concomitantly, parenting also limits negative affective states in adults, which emerge when the coping with neonatal distress becomes a challenge. Whether neural circuits that process negative affect orchestrate components of parental behaviours remains, however, unknown. Here, we identify functional and transcriptional signatures of parental behaviours in neurons of the negative emotion center lateral habenula receiving bed nucleus of the stria terminalis innervation (BNSTLHb). Calcium imaging and optogenetic manipulations reveal that LHb neurons of virgin female mice increase their activity in response to pup distress vocalization and are necessary for pup calls-driven aversive behaviours. Accordingly, LHb activity rises during the retrieval of a pup to a nest, a behaviour worsened by optogenetic LHb inactivation. Intersectional cell identification and transcriptional profiling associates the BNSTLHb neuronal population to parental behaviours and outlines gene expression in female virgins that is similar to mothers but divergent from non-parental virgin male mice. Accordingly, optogenetic activation and inactivation of the BNST-LHb pathway maximizes and suppresses, respectively, the parental behaviour. Finally, tracking and manipulating single BNSTLHb cell activity demonstrates specificity of this neuronal subset for encoding negative affect and pup retrieval, but not sociability amongst conspecifics. Thus, BNSTLHb cells are operational for female parenting, demonstrating that a negative affect neural circuit processes newborn distress signals and limits them through female parenting.
Overall design Single cell RNA sequencing (scRNA-seq) on sorted KASH-GFP+ BNSTLHb from three categories, namely virgin females, sexually-experienced females (mothers) and virgin male mice.
Contributor(s) Lecca S, Congiu M, Royon L, Restivo L, Girard B, Mazaré N, Bellone C, Telley L, Mameli M
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Submission date Jan 03, 2023
Last update date Jan 05, 2023
Contact name Ludovic Telley
Organization name UNIL
Street address Department of Biomedical Sciences University of Lausanne Rue du Bugnon 9
City Lausanne
ZIP/Postal code 1005
Country Switzerland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (3)
GSM6914115 MAc_S1_L001_R1_001
GSM6914116 MBc_S1_L001_R1_001
GSM6914117 MCc_S1_L001_R1_001
BioProject PRJNA917559

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Supplementary file Size Download File type/resource
GSE222064_all_unap.Rds.gz 38.8 Mb (ftp)(http) RDS
GSE222064_habenula.integrated.Rds.gz 1015.1 Mb (ftp)(http) RDS
GSE222064_singlecell_countmatrix.tsv.gz 2.7 Mb (ftp)(http) TSV
GSE222064_singlecell_metadata.tsv.gz 21.9 Kb (ftp)(http) TSV
GSE222064_source.R.gz 7.1 Kb (ftp)(http) R
GSE222064_src.R.gz 4.7 Kb (ftp)(http) R
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Raw data are available in SRA
Processed data are available on Series record

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