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Status |
Public on Jan 04, 2023 |
Title |
A neural substrate for negative affect dictates female parental behaviour |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Parental behaviours are essential to secure survival and wellbeing of newborns. Concomitantly, parenting also limits negative affective states in adults, which emerge when the coping with neonatal distress becomes a challenge. Whether neural circuits that process negative affect orchestrate components of parental behaviours remains, however, unknown. Here, we identify functional and transcriptional signatures of parental behaviours in neurons of the negative emotion center lateral habenula receiving bed nucleus of the stria terminalis innervation (BNSTLHb). Calcium imaging and optogenetic manipulations reveal that LHb neurons of virgin female mice increase their activity in response to pup distress vocalization and are necessary for pup calls-driven aversive behaviours. Accordingly, LHb activity rises during the retrieval of a pup to a nest, a behaviour worsened by optogenetic LHb inactivation. Intersectional cell identification and transcriptional profiling associates the BNSTLHb neuronal population to parental behaviours and outlines gene expression in female virgins that is similar to mothers but divergent from non-parental virgin male mice. Accordingly, optogenetic activation and inactivation of the BNST-LHb pathway maximizes and suppresses, respectively, the parental behaviour. Finally, tracking and manipulating single BNSTLHb cell activity demonstrates specificity of this neuronal subset for encoding negative affect and pup retrieval, but not sociability amongst conspecifics. Thus, BNSTLHb cells are operational for female parenting, demonstrating that a negative affect neural circuit processes newborn distress signals and limits them through female parenting.
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Overall design |
Single cell RNA sequencing (scRNA-seq) on sorted KASH-GFP+ BNSTLHb from three categories, namely virgin females, sexually-experienced females (mothers) and virgin male mice.
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Contributor(s) |
Lecca S, Congiu M, Royon L, Restivo L, Girard B, Mazaré N, Bellone C, Telley L, Mameli M |
Citation missing |
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Submission date |
Jan 03, 2023 |
Last update date |
Jan 05, 2023 |
Contact name |
Ludovic Telley |
E-mail(s) |
ludovic.telley@unil.ch
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Organization name |
UNIL
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Street address |
Department of Biomedical Sciences University of Lausanne Rue du Bugnon 9
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City |
Lausanne |
ZIP/Postal code |
1005 |
Country |
Switzerland |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (3) |
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Relations |
BioProject |
PRJNA917559 |
Supplementary file |
Size |
Download |
File type/resource |
GSE222064_all_unap.Rds.gz |
38.8 Mb |
(ftp)(http) |
RDS |
GSE222064_habenula.integrated.Rds.gz |
1015.1 Mb |
(ftp)(http) |
RDS |
GSE222064_singlecell_countmatrix.tsv.gz |
2.7 Mb |
(ftp)(http) |
TSV |
GSE222064_singlecell_metadata.tsv.gz |
21.9 Kb |
(ftp)(http) |
TSV |
GSE222064_source.R.gz |
7.1 Kb |
(ftp)(http) |
R |
GSE222064_src.R.gz |
4.7 Kb |
(ftp)(http) |
R |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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