GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE22178 Query DataSets for GSE22178
Status Public on Oct 07, 2010
Title Combinatorial Transcriptional Control in Blood Stem/Progenitor Cells: Genome-wide Analysis of 10 major Transcriptional Regulators
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Combinatorial transcription factor (TF) interactions control cellular phenotypes and therefore underpin stem cell formation, maintenance and differentiation. Here we report the genome-wide binding patterns and combinatorial interactions for 10 key regulators of blood stem/progenitor cells (Scl/Tal1, Lyl1, Lmo2, Gata2, Runx1, Meis1, Pu.1, Erg, Fli-1, Gfi1b) thus providing the most comprehensive TF dataset for any adult stem/progenitor cell type to date. Genome-wide computational analysis of complex binding patterns followed by functional validation revealed the following: First, a previously unrecognized combinatorial interaction between a heptad of TFs (Scl, Lyl1, Lmo2, Gata2, Runx1, Erg, Fli-1). Second, we implicate direct protein-protein interactions between four key regulators (Runx1, Gata2, Scl, Erg) in stabilising complex binding to DNA. Third, Runx1+/-::Gata2+/- compound heterozygous mice are not viable with severe haematopoietic defects at midgestation. Taken together, this study demonstrates the power of genome-wide analysis in generating novel functional insights into the transcriptional control of stem and progenitor cells.
Overall design 10 Samples (9 Transcription Factors and 1 Histone Modification) and 1 Control (IgG). All from the same cell line, a haematopoietic progenitor cell line (HPC-7).
Contributor(s) Wilson NK, Foster SD, Wang X, Knezevic K, Schütte J, Kinston S, Ouwehand WH, Dzierzak E, Pimanda JE, de Bruijn MF, Göttgens B
Citation(s) 20887958, 21576367
Submission date Jun 07, 2010
Last update date May 15, 2019
Contact name Samuel David Foster
Organization name Cambridge Institute for Medical Research
Department Department of Haematology
Lab Haematopoietic Stem Cell Lab
Street address Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road
City Cambridge
State/province Cambridgeshire
ZIP/Postal code CB2 0XY
Country United Kingdom
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (11)
GSM552232 HPC7_H3AcK9
GSM552233 HPC7_Fli1
GSM552234 HPC7_Gata2
SRA SRP002575
BioProject PRJNA127555

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22178.tar.gz 5.4 Mb (ftp)(http) TAR
GSE22178_RAW.tar 5.3 Gb (http)(custom) TAR (of BEDGRAPH, TXT)
GSE22178_README.txt 3.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap