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| Status |
Public on Apr 04, 2024 |
| Title |
Extraembryonic gut endoderm cells undergo programmed cell death during development (scRNA-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Despite a distinct developmental origin, extraembryonic cells in mice contribute to gut endoderm and converge to transcriptionally resemble their embryonic counterparts. Notably, extraembryonic progenitors share a non-canonical epigenome, raising several pertinent questions, including whether this landscape is reset to match the embryonic regulation and if these cells persist into later development. Here, we developed a two-color lineage tracing strategy to track and isolate extraembryonic cells over time. We find that extraembryonic gut cells display substantial memory of their developmental origin including retention of their original DNA methylation landscape and resulting transcriptional signatures. Furthermore, we show that extraembryonic gut cells undergo programmed cell death and neighboring embryonic cells clear their remnants via non-professional phagocytosis. By midgestation, we no longer detect extraembryonic cells in the wild type gut while they persist and differentiate further in p53 mutant embryos. Our study provides key insights into the molecular and developmental fate of extraembryonic cells inside the embryo.
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| Overall design |
Single-cell RNA-seq profiling of sorted gut endoderm cells with embryonic and extraembryonic origin.
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| Contributor(s) |
Batki J, Hetzel S, Schifferl D, Bolondi A, Walther M, Wittler L, Grosswendt S, Herrmann BG, Meissner A |
| Citation(s) |
38849542 |
| |
| Submission date |
Dec 19, 2022 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Sara Hetzel |
| E-mail(s) |
hetzel@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Genome Regulation
|
| Lab |
Meissner Lab
|
| Street address |
Ihnestraße 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (3) |
| GSM6859078 |
scRNA_E95_mCherry_and_GFP_mCherry_dual |
| GSM7975176 |
scRNA_E135_gastrointestinal_tract_EPCAM_WT |
| GSM7975177 |
scRNA_E135_gastrointestinal_tract_EPCAM_Trp53KO |
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| This SubSeries is part of SuperSeries: |
| GSE250084 |
Extraembryonic gut endoderm cells undergo programmed cell death during development |
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| Relations |
| BioProject |
PRJNA913958 |
