|
| Status |
Public on Dec 22, 2022 |
| Title |
Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants. |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To investingate cell types in wild-type zebrafish explants.
Methods: Wild-type explants (injected with phenol red) were harvested at 10hpf. Libraries were prepared using Chromium Controller and Chromium Single Cell 3’Library & Gel Bead Kit v3 (10x Genomics, PN-1000075) according to the manufacturer’s protocol for 10000 cells recovery.
Results: A total of 9,968 single-cell transcriptomes were collected after stringent quality control measures.
Conclusions: 6 Cell types were identified, wild-type explants mainly contains the anterior neural ectoderm and epidermis at 10 hpf.
|
| |
|
| Overall design |
Wild-type zebrafish explants were harvested at 10hpf for scRNA-seq.
|
| |
|
| Contributor(s) |
Cheng T, Xing Y |
| Citation(s) |
37729057 |
| |
| Submission date |
Dec 17, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Tao cheng |
| E-mail(s) |
11818118@zju.edu.cn
|
| Phone |
0571-88208276
|
| Organization name |
Zhejiang University
|
| Department |
Institute of genetics
|
| Street address |
886 Yuhangtang Road
|
| City |
Hang Zhou |
| State/province |
Zhe Jiang |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA913420 |
