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Series GSE221137 Query DataSets for GSE221137
Status Public on Jun 05, 2024
Title ATAC-seq analyses of AR-V7.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Although AR-V7 has been intensively studied, it remains unclear whether AR-V7 and other AR splicing variants can specifically activate a distinctive transcriptional program from the full-length AR (AR-FL), and whether AR-V7 may play a role in accelerating the metastatic progression of castration-resistant PCa (CRPC). In this study, we hypothesize that AR-V7 can drive a distinct transcription program from AR-FL in CRPC condition. To test this, we performed ATAC-seq using LNCaP model with inducible overexpression of AR-V7 to examine the function of AR-V7. We demonstrated that AR-V7 has “pioneer factor-like” activities to access the androgen-responsive elements (AREs) located at compact chromatin regions. More importantly, we found that SOX9, a critical metastasis driver gene, was a direct target and downstream effector of AR-V7, and its protein expression was dramatically upregulated at AR-V7-induced bone lesions.
 
Overall design ATAC-seq analyses in LNCaP table cells with or without overexpression of AR-V7 and stimulated with or without DHT under 5% charcoal-stripped FBS condition. The stable cells were generated by stably infecting LNCaP cells with tetracycline inducible overexpression of wild-type AR-V7 virus. These stable cells were grown in the medium containing 10% tetracycline-free FBS.
 
Contributor(s) Han D, Labaf M, Zarringhalam K, Cai C
Citation(s) 38687617
Submission date Dec 16, 2022
Last update date Jun 05, 2024
Contact name Maryam Labaf
E-mail(s) maryam.labaf001@umb.edu
Organization name University of Massachusetts Boston
Department Biology
Lab Cai Lab
Street address 100 William T Morrissey Blvd
City Boston
State/province MA
ZIP/Postal code 02125
Country USA
 
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (8)
GSM6845871 ATAC_LN-tet-ARV7_CSS_Veh_rep1
GSM6845872 ATAC_LN-tet-ARV7_CSS_Veh_rep2
GSM6845873 ATAC_LN-tet-ARV7_CSS_Dox_rep1
This SubSeries is part of SuperSeries:
GSE221142 Distinct activity of androgen receptor splice variants in promoting prostate cancer metastasis
Relations
BioProject PRJNA913099

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE221137_ATAC_LN-tet-ARV7_CSS_DHT.bw 200.9 Mb (ftp)(http) BW
GSE221137_ATAC_LN-tet-ARV7_CSS_DHT.narrowPeak.gz 2.6 Mb (ftp)(http) NARROWPEAK
GSE221137_ATAC_LN-tet-ARV7_CSS_Dox.bw 210.9 Mb (ftp)(http) BW
GSE221137_ATAC_LN-tet-ARV7_CSS_Dox.narrowPeak.gz 2.1 Mb (ftp)(http) NARROWPEAK
GSE221137_ATAC_LN-tet-ARV7_CSS_Dox_DHT.bw 404.0 Mb (ftp)(http) BW
GSE221137_ATAC_LN-tet-ARV7_CSS_Dox_DHT.narrowPeak.gz 1.9 Mb (ftp)(http) NARROWPEAK
GSE221137_ATAC_LN-tet-ARV7_CSS_Veh.bw 318.2 Mb (ftp)(http) BW
GSE221137_ATAC_LN-tet-ARV7_CSS_Veh.narrowPeak.gz 2.3 Mb (ftp)(http) NARROWPEAK
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