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Series GSE221027 Query DataSets for GSE221027
Status Public on Dec 15, 2022
Title Human PSEN1 Mutant Glia Improve Spatial Learning and Memory in Aged Mice
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PSEN1 ΔE9 mutation causes a familial form of Alzheimer's disease. We have previously shown that human induced pluripotent stem cell (iPSC)-derived astrocytes carrying PSEN1 ΔE9 mutation exhibit transcriptional and functional abnormalities (Stem Cell Reports. 2017;9:1885-1897). Here we injected glial progenitors derived from 2 pairs of PSEN1 ΔE9 mutant- isogenic CTRL iPSCs intracerebroventricularly into newborn mice. At the age of 18 months, mouse hippocampi containing human cells (5 x PSEN1 ΔE9 + 6 x CTRL, all from male mice) were quickly dissected out and bulk RNA sequencing analysis was performed. Our results shed more light on the efffect of PSEN1 ΔE9 mutation on human glia in vivo as well as on the effect of the presence of mutant human glia on the surrounding healthy mouse cells.
Overall design Human iPSC-derived glial progenitors were injected intracerebroventricularly into newborn immunedeficient mice and were let to proliferate and maturate in the mouse brain for 18 months. Ribo-depleted RNA was then sequenced with Illumina Next-seq 500 from mouse hippocampi containing human cells (5 x PSEN1 ΔE9 + 6 x CTRL). Sequences from human and mouse origin were analyzed separately.
Contributor(s) Koistinaho J, Rolova T
Citation(s) 36552881
Submission date Dec 15, 2022
Last update date Mar 16, 2023
Contact name Iiris Hovatta
Organization name University of Helsinki
Department Department of Psychology and Logopedics
Lab Neurogenomics
Street address Haartmaninkatu 3
City Helsinki
ZIP/Postal code 00014
Country Finland
Platforms (1)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
Samples (11)
GSM6840192 54_S4
GSM6840193 56_S6
GSM6840194 57_S7
BioProject PRJNA912624

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Supplementary file Size Download File type/resource
GSE221027_counts_AD-astro_hsa.txt.gz 377.7 Kb (ftp)(http) TXT
GSE221027_counts_AD-astro_mmu.txt.gz 585.0 Kb (ftp)(http) TXT
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