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| Status |
Public on Sep 11, 2023 |
| Title |
Discovery of extracellular vesicles derived miR-185-5p in patient’s plasma as an indicator for advanced adenoma and malignant tumor of colon |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We attempted to screen and identify new plasma EV delivered microRNA can simultaneously diagnose AA, early CRC and advanced CRC in healthy people. We employed microRNA deep sequencing assay for the primary screen and performed TaqMan microRNA assay for further test and validation. Through stepwise screen and validation in two independent cohorts (cohort I: n=75, cohort II: n=104), we demonstrated that plasma EV-delivered miR-185-5p is a potential indicator of AA and CRC in Chinese patients. In the supplementary phase, we also preliminarily proved that miR-185-5p can promote the development of CRC in vivo and in vitro.
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| Overall design |
Comparative miRNA expression profiling analysis of miRNA-seq data for plasma extracellular vesicles in healthy donor, advanced adenoma, and colorectal cancer patients.
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| Contributor(s) |
Shi Y, Li X, Hu Z, Wang H |
| Citation(s) |
37386465 |
| |
| Submission date |
Dec 07, 2022 |
| Last update date |
Sep 12, 2023 |
| Contact name |
yunjie shi |
| E-mail(s) |
shiyunpengjie@126.com
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| Phone |
8613817955053
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| Organization name |
Naval Medical University
|
| Street address |
No 800，Xiangyin Road
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| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA909776 |
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