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Status |
Public on Aug 08, 2023 |
Title |
Siginificance of Saccharomyces cerevisiae Cdc55p on gene expression at initial stage of alcoholic fermentation |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Our previous report revealed that protein phosphatase 2A (PP2A), complexed with the B55delta-type regulatory subunit (i.e. Cdc55p), is solely responsible for the outstanding glycolytic activity of sake yeast strains (Watanabe et al., Appl. Environ. Microbiol. 85, e02083-18 (2019). However, how PP2A mediates yeast alcoholic fermentation remains elusive. Thus, RNA-seq analysis of S. cerevisiae cdc55-delta cells at the initial fermentation stage was performed to identify the downstream effector targeting the glycolytic control.
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Overall design |
Cdc55p-deficient cells (cdc55-delta) and their control (wild type) of S. cerevisiae BY4741 strain at 6 h after inoculation in YPD20 medium were collected and subjected to RNA-seq analysis.
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Contributor(s) |
Watanabe D |
Citation(s) |
37479699 |
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Submission date |
Dec 05, 2022 |
Last update date |
Aug 08, 2023 |
Contact name |
Daisuke Watanabe |
E-mail(s) |
watanabe.daisuke@bs.naist.jp
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Organization name |
Nara Institute of Science and Technology
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Street address |
8916-5 Takayamacho
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City |
Ikoma |
State/province |
Nara |
ZIP/Postal code |
630-0192 |
Country |
Japan |
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Platforms (1) |
GPL30266 |
DNBSEQ-G400 (Saccharomyces cerevisiae) |
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Samples (2) |
GSM6784608 |
Wild-type yeast, 6 h in fermentative medium, rep1 |
GSM6784609 |
Cdc55p-deficient yeast, 6 h in fermentative medium, rep1 |
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Relations |
BioProject |
PRJNA908765 |