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Series GSE218582 Query DataSets for GSE218582
Status Public on Nov 21, 2023
Title Single-cell transcriptional analysis of developmental maturation strategies for human pluripotent stem cell-derived heart organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human pluripotent stem cells possess the ability to recapitulate key events of mammalian organogenesis in vitro, including heart development. Previously-described human heart organoid protocols elicit early embryonic-like cardiac phenotypes and morphologies. We hypothesized that human heart organoids can be made significantly more complex and physiologically relevant through the implementation of in utero gestational biochemical phenomena. Here, we designed and applied multiple developmental maturation strategies on our human heart organoids for a period of 10 days. Our data reveals the emergence of atrial and ventricular cardiomyocyte populations, valvular cells, epicardial cells, proepicardial-derived cells, endothelial cells, stromal cells, conductance cells, and cardiac progenitors, all of them cell types present in the primitive heart tube.
Overall design Single-cell RNA-sequencing of various developmentally maturated human heart organoids
Contributor(s) Aguirre A, Volmert B, Riggs A, Kiselev A, Park S
Citation(s) 38086920
Submission date Nov 22, 2022
Last update date Jan 02, 2024
Contact name Aitor Aguirre
Phone 5173547072
Organization name Michigan State University
Department Biomedical Engineering
Lab Aguirre Lab
Street address 775 Woodlot Dr
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM6752835 Control, day 34
GSM6752836 MM, day 34
GSM6752837 EMM1, day 34
BioProject PRJNA904361

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Supplementary file Size Download File type/resource
GSE218582_RAW.tar 57.2 Gb (http)(custom) TAR (of CLOUPE, MTX, TAR, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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