GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE21837 Query DataSets for GSE21837
Status Public on May 14, 2010
Title Expression data from unactivated vs. activated PBMCs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Long-lasting activation of T cells requires up-regulation of many genes, for example of transcription factors, cytoskeletal proteins and cell surface proteins encluding ion channels. An increase of ion channel density at the cell surface reflects the needs to manage increased Ca2+ influx into the activated T cell. Using oligonucleotide-based arrays we have surveyed changes in ion channel mRNA expression that occur upon T cell activation. We used Affymetrix Analysis to confirmate our data achieved by self-designed glass array analysis.
Overall design We isolated PBMCs from blood of healthy donors and divided those into two parts. One was activated for 72 hr by adding PHA-L whereas the other part was incubated in the same way but not stimulated.
Contributor(s) Ogrodowczyk C, Pongs O
Citation(s) 25181038
Submission date May 14, 2010
Last update date Dec 06, 2018
Contact name Christoph Ogrodowczyk
Organization name ZMNH
Department Institute for Neural Signal Transduction
Street address Falkenried 94
City Hamburg
ZIP/Postal code 20251
Country Germany
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM543433 PBMCs rep.1 unactivated
GSM543434 PBMCs rep 2 unactivated
GSM543435 PBMCs rep 3 unactivated
BioProject PRJNA126921

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21837_Complete_Analysis_Data.txt.gz 4.1 Mb (ftp)(http) TXT
GSE21837_RAW.tar 11.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap