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Series GSE218160 Query DataSets for GSE218160
Status Public on Nov 17, 2022
Title miR expression microarray analysis on M2 macrophages treated with epigenetic modifiers
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Epigenetic regulation of gene expression involves DNA methylation, histone methylation or acetylation, and microRNA (miRNA)-mediated mRNA degradation.
We studied, if epigenetic modifiers namely 5-aza-2'-deoxycytidine and trichostatin A could remodulate the miR expression in M2 macrophage and enable M2-M1 reprogramming.
 
Overall design Bone marrow cells were isolated from tibias and femurs of Balb/c mice and cultured in DMEM supplemented with 10 ng/mL of colony-stimulating factor-1 (CSF-1; Gibco, Carlsbad, CA, USA) and 10% FBS for 7 d. The culture medium was changed every other day. Next, the cells were incubated with 100 ng/mL of lipopolysaccharide and 20 ng/mL of recombinant mouse IFN-γ (R&D Systems, Minneapolis, MN, USA), for 24–48 h to achieve M1 polarization. For M2 polarization, BMDMs were incubated with 20 ng/mL of recombinant mouse IL-4 for 24–48 h. M2-polarized macrophages were treated with either 50 nM 5-aza-dC (Merck, Rahway, NJ, USA) for 72 h or 25 nM TSA (Merck) for 48 h. For treatment with the combination, M2-polarized macrophages were treated with 5-aza-dC for 24 h followed by treatment with 5-aza-dC and TSA for 48 h.RNAs for miRNA profiling were isolated using the miRNeasy® Mini Kit. Microarray analysis of miRNAs was performed using an Affymetrix GeneChip miRNA 4.0 array (Affymetrix, Santa Clara, CA, USA) by Macrogen (Seoul, Korea).
 
Contributor(s) Lee B, Vadevoo PV, Gowry GG
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Submission date Nov 17, 2022
Last update date Nov 20, 2022
Contact name Byungheon Lee
E-mail(s) leebh@knu.ac.kr
Phone +82-10-5501-3457
Organization name Kyungpook National University
Department School of Medicine
Lab Department of Biochemistry and Cell Biology
Street address 680 Guckchaebosang-ro, Jung-gu
City Daegu
State/province Outside U.S. & Canada
ZIP/Postal code 41944
Country South Korea
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (7)
GSM6735734 Control BMDM
GSM6735735 M1, biological rep 1
GSM6735736 M1, biological rep 2
Relations
BioProject PRJNA902567

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218160_RAW.tar 5.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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