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| Status |
Public on May 14, 2024 |
| Title |
ILC2 development requires GATA3-related super-enhancers after lineage commitment |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
This SuperSeries is composed of the SubSeries listed below.
Group 2 innate lymphoid cells (ILC2) are a subset of innate lymphocytes that produce type 2 cytokines, including IL-4, IL-5, and IL-13. GATA3 being a critical transcription factor for ILC2 development at multiple stages. However, when and how GATA3 is induced to the levels required for ILC2 development remains elusive. Herein, we identified ILC2-specific tandem GATA3-related super-enhancers (G3SE) that induce high GATA3 in ILC2-committed precursors. G3SE-deficient mice exhibit ILC2 loss in the BM, lung, liver, and small intestine, a slight ILC1 increase, and no difference in ILC3, LTi, NK cells, nor Th2 cells. Single-cell RNA-sequencing revealed that GATA3 induction in late ILC2-committed precursors depends on G3SEs and is required for the transition to mature ILC2. Cnot6l, part of the CCR4-Not deadenylase complex, is a possible GATA3 target during ILC2 development. Our findings indicate a novel regulatory mechanism for GATA3 expression during ILC2 development.
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| Overall design |
Refer to individual Series
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| Citation(s) |
38969652, 38923989 |
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| Submission date |
Nov 16, 2022 |
| Last update date |
Aug 08, 2024 |
| Contact name |
Arifumi Iwata |
| Organization name |
Chiba University
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| Department |
Department of Allergy and Clinical Immunology
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| Street address |
1-8-1 Inohana, Chuou-ku
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| City |
Chiba-shi |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (3) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (17)
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| This SuperSeries is composed of the following SubSeries: |
| GSE218142 |
ATAC-seq of bone marrow ILC2 lineages and Lung ILC2 steady state |
| GSE218144 |
H3K27ac ChIP-seq for lung ILC2 and CD4 T cells in HDM induced asthma |
| GSE218154 |
Single cell RNA sequencing for α lymphoid progenitor |
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| Relations |
| BioProject |
PRJNA902420 |
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