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| Status |
Public on May 29, 2024 |
| Title |
Integrated transcriptomics of human blood vessels defines the spatially regulated niche for early mesenchymal progenitors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Blood vessel walls are organized into concentric cellular layers, in which the outer layer, or tunica adventitia, represents the major niche for mesenchymal progenitor cells. By profiling large vessels (arteries and veins) using spatial transcriptomics, we found a hierarchy of stem cells based on spatial organization. In addition, we performed scRNaseq on CD34+ adventitial cells and integrated to out spatial transcriptomics where we identified the novel stem cell marker CD201 in the outer layer. This segregation was regulated by the WNT and TGFβ signaling pathways, which was confirmed by total RNAseq of CD201Low/High subsets of adventitial cells. To investigate the transcriptomic differences between the novel subset of of adventitial cells (CD34+,CD31-,CD45-) based on CD201 expression, FACTS isolated adventitial cells with low /high CD201 expression were sequenced.
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| Overall design |
subsets of adipose derived adventitial cells (CD34+,CD31-,CD45-) with low or high expression of CD201 were isolated by by Fluorescence-activated cell sorting (FACS) based on their immunophenotype and analyzed using total RNA sequencing. ANOVA comparison was performed between three biologic replicates of each class.
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| Contributor(s) |
Wang Y, Thottappillil N, Gomez-Salazar MA, James AW |
| Citation(s) |
39025061 |
| |
| Submission date |
Nov 03, 2022 |
| Last update date |
Sep 12, 2024 |
| Contact name |
Aaron James |
| E-mail(s) |
geo.jameslab@gmail.com
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| Phone |
4105021579
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| Organization name |
Johns Hopkins University
|
| Department |
Pathology
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| Lab |
James Lab
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| Street address |
720 Rutland Avenue, Ross Bldg., Room 529
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| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA897919 |
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