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Series GSE217093 Query DataSets for GSE217093
Status Public on Jan 01, 2024
Title RNAseq and scRNAseq analysis of UPFL mouse tumors
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fibroblast growth factor receptor 3 (FGFR3) is altered in up to 50% of urothelial cancers (UC), yet its functional impact is not fully understood. We generated a Cre-inducible FGFR3 S249C allele (LSL-FGFR3 S249C) to understand the functional impact of FGFR3 activation on UC biology, the immune microenvironment, and how FGFR inhibition combines with PD1 immune checkpoint inhibition (ICI). Uroplakin3a (Upk3a) driven expression of Trp53 R270H and FGFR3 S249C (UPFL) promoted tumor formation.
Overall design bulk RNAseq from primary UPFL tumors (n=7) and scRNAseq from primary UPFL control (n=2) or Erdafitinib (n=2) treated tumors.
Pooled scraped urothelium from 10 wild-type female mice without treatment are anlyzed using scRNAseq
Contributor(s) Okato A, Utsumi T, Ranieri M, Zheng X, Zhou M, Pereira LD, Chen T, Wu D, Manocha U, Wobker SE, Damrauer JS, Wong K, Kim WY
Citation(s) 38226620
Submission date Nov 02, 2022
Last update date Jan 29, 2024
Contact name William Kim
Organization name UNC Chapel Hill
Department Lineberger Comprehensive Cancer Center
Street address 450 West Dr
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM6704993 UPFL1
GSM6704994 UPFL2
GSM6704995 UPFL3
BioProject PRJNA896928

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE217093_RAW.tar 164.7 Mb (http)(custom) TAR (of H5)
GSE217093_norm.mTumors.count.UPFL.txt.gz 682.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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