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Series GSE217012 Query DataSets for GSE217012
Status Public on Sep 27, 2023
Title MYD88 dimerization controls the proliferative, invasive, and pro-inflammatory responses of synovial fibroblasts from rheumatoid arthritis patients
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We analyzed RNA-seq from rheumatoid arthritis synovial fibroblats and osteoarthritis syniovial fibroblast patients. Our goal was to evaluate the gene expression signatures after treatment with the MyD88 dimerization onhibitor ST2825 and identify its therapeutic potential.
Methods: We analyzed bulk RNA-seq from RA synovial fibroblasts in diferent conditions: Untreated, ST2825-treated, LPS-treated, LPS+ST2825-treated. Aditionally, we analyzed bulk RNA-seq from OA synovial fibroblatss as a control. Collection of RNA was performed after 24 h of treatment and samples were sequenced to determine transcriptional changes and regulated processes.
Results: paired-end reads were mapped to the homo sapiens GRCh37 genome assembly. RNA levels were normalized by RPKM and student's t-test.
Conclusion: The aggressiveness of the RA SFs, in terms of their proliferation, invasiveness, and increased expression of pain and inflammation mediators was decreased by ST2825. Our data strongly suggest that targeting MyD88 dimerization could mitigate joint inflammation in RA SFs.
Overall design Identification of the potential effect of the MyD88 dimerisation inhibitor ST2825 on pathogenic expression signatures to modulate joint inflammatory processes in RA patients.
Web link
Contributor(s) Ramirez-Perez S, Bhattaram P
Citation(s) 37749630
Submission date Nov 01, 2022
Last update date Dec 28, 2023
Contact name Sergio Ramirez-Perez
Organization name Emory University
Department Orthopaedics
Lab Bhattaram Lab
Street address 615 Michael St NE
City Atlanta
State/province Georgia
ZIP/Postal code 30322
Country USA
Platforms (2)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (15)
GSM6703567 RA_FLS1_UT
GSM6703568 RA_FLS3_UT
GSM6703569 RA_FLS4_UT
BioProject PRJNA896568

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE217012_Normalized_RPKM_LOG2_matrix.txt.gz 124.2 Kb (ftp)(http) TXT
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Processed data are available on Series record

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