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| Status |
Public on Feb 01, 2023 |
| Title |
Single-cell epigenomics and high-density genetic variation resolve the architecture of the Treg regulatory network [scATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To systematically examine the transcription factors and cis-regulatory elements that shape diverse Treg regulatory programs, we used single-cell Assay for Transposase-Accessible Chromatin with high-throughput sequencing (scATAC-seq) to generate chromatin accessibility profiles of mouse regulatory and conventional T cells from different organs and genetic backgrounds.
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| Overall design |
T regulatory (Treg) and conventional (Tconv) cells were sorted from either different organs (spleen, colon), genetic backgrounds (B6, B6/Cast F1), or different transcription factor knockouts (FoxP3, Gata3) for analysis by scATAC-seq
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| Contributor(s) |
Chowdhary K, Mathis D, Benoist C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 31, 2022 |
| Last update date |
Feb 03, 2023 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA896167 |
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