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Series GSE21688 Query DataSets for GSE21688
Status Public on May 15, 2010
Title Transcript profiling of individual twin blastomeres derived by splitting 2-cell stage murine embryos
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content. Amplified mRNA from 12 blastomeres derived from six embryos approximately mid-way through their second cell cycle was analyzed. Probes displaying normalized values greater than 0.25 were selected and examined for consistent bias in expression within blastomere pairs. Although transcript content varied both between individual embryos and twin blastomeres, no consistent asymmetries were observed for the majority of genes. On the other hand, 769 genes displayed a greater than 1.4-fold difference in expression across 5 of 6 pairs of blastomeres and 178 genes differed across all 6 pairs. These genes separated into two groups by class discovery clustering. Of the 769 differentially expressed genes, 163 were significantly up- or down-regulated in one sister blastomere compared to the other. Transcripts encoding proteins implicated in RNA processing and cytoskeletal organization were highly represented among the most abundant, differentially distributed mRNA. We conclude that there are many differences that distinguish twin blastomeres derived from a single 2-cell stage embryo but that only a few of these differences are consistent across multiple pairs of embryos. We hypothesize that a stochastically-based lack of synchrony in cell cycle progression between the two cells might explain some or all of the asymmetries in transcript composition.
Overall design 6 pairs of blastomeres were analyzed for a total of 12 samples.
Contributor(s) Roberts RM, Katayama M, Magnuson S, Falduto MT, Torres KE
Citation(s) 21076082
Submission date May 05, 2010
Last update date Jan 12, 2017
Contact name Michael Falduto
Phone 847-291-9602
Organization name GenUs BioSystems, Inc.
Street address 1808 Janke, Unit M
City Northbrook
State/province IL
ZIP/Postal code 60062
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM541143 Experiment 1, Blastomere pair D, Cell 1
GSM541144 Experiment 1, Blastomere pair D, Cell 2
GSM541145 Experiment 1, Blastomere pair I, Cell 1
BioProject PRJNA125853

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Supplementary file Size Download File type/resource
GSE21688_RAW.tar 103.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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