|
| Status |
Public on May 30, 2010 |
| Title |
Diverse Targets of the Transcription Factor STAT3 Contribute to T Cell Pathogenicity and Homeostasis [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
STAT3, an essential transcription factor with pleiotropic functions, plays critical roles in the pathogenesis of autoimmunity. Despite recent data linking STAT3 with inflammatory bowel disease, exactly how it contributes to chronic intestinal inflammation is not known. Using a T cell transfer model of colitis we found that STAT3 expression in T cells was essential for the induction of both colitis and systemic inflammation. STAT3 was critical in modulating the balance of T helper 17 (Th17) and regulatory T (Treg) cells, as well as in promoting CD4+ T cell proliferation. We used chromatin immunoprecipitation and massive parallel sequencing (ChIP-Seq) to define the genome-wide targets of STAT3 in CD4+ T cells. We found that STAT3 bound to multiple genes involved in Th17 cell differentiation, cell activation, proliferation and survival, regulating both expression and epigenetic modifications. Thus, STAT3 orchestrates multiple critical aspects of T cell function in inflammation and homeostasis.
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| Overall design |
Examination of gene expression profiles and histone modification (H3K4me3) in wild type and STAT3-deficient T cells. Also Examination of STAT3 binding in wild type T cells.
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| Contributor(s) |
Durant L, Watford WT, Ramos HL, Laurence A, Vahedi G, Wei L, Takahashi H, Sun H, Kanno Y, Powrie F, O’Shea JJ |
| Citation(s) |
20493732 |
| |
| Submission date |
May 05, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Golnaz Vahedi |
| Organization name |
National Institutes of Health
|
| Department |
NIAMS
|
| Lab |
Lymphocyte Cell Biology Section
|
| Street address |
9000 Rockville Pike Bldg 10 Rm 13C101A
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (3) |
| GSM540720 |
Stat3fl/fl FoxP3-GFP<STAT3 wild type>, H3K4me3_ChIPSeq |
| GSM540721 |
Stat3fl/fl FoxP3-GFP, CD4-Cre<STAT3 deficient>, H3K4me3_ChIPSeq |
| GSM540722 |
Stat3fl/fl FoxP3-GFP<STAT3 wild type>, STAT3-ChIPSeq |
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| This SubSeries is part of SuperSeries: |
| GSE21671 |
Diverse Targets of the Transcription Factor STAT3 Contribute to T Cell Pathogenicity and Homeostasis |
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| Relations |
| SRA |
SRP002451 |
| BioProject |
PRJNA129289 |
