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| Status |
Public on May 29, 2024 |
| Title |
Polycomb sustains promoters in a deep OFF-state by limiting PIC formation to counteract transcription |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The Polycomb system plays fundamental roles in regulating gene expression during mammalian development. However, how it controls transcription to enable gene repression has remained enigmatic. Here we employ rapid degron-based depletion coupled with live-cell transcription imaging and single-particle tracking to uncover how the Polycomb system controls transcription in single cells. We discover that the Polycomb system is not a constitutive block to transcription but instead sustains a long-lived deep promoter OFF-state which limits the frequency with which the promoter can enter into a transcribing state. We demonstrate that Polycomb sustains this deep promoter OFF-state by counteracting the binding of factors that enable early transcription pre-initiation complex formation and show that this is necessary for gene repression. Together these important discoveries provide a new rationale for how the Polycomb system controls transcription and suggests a universal mechanism that could enable the Polycomb system to constrain transcription across diverse cellular contexts.
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| Overall design |
The genomic distribution of TAF1-T7 was profiled in RING1B-miniAID;TAF1-T7-dTAG mESCs, in which RING1B can be conditionally depleted.
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| Contributor(s) |
Szczurek A, Dimitrova E, Klose RJ |
| Citation missing |
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| |
| Submission date |
Oct 26, 2022 |
| Last update date |
May 30, 2024 |
| Contact name |
Emilia Dimitrova |
| E-mail(s) |
emilia.dimitrova@bioch.ox.ac.uk
|
| Organization name |
University of Oxford
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| Department |
Biochemistry
|
| Lab |
Rob Klose
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA894545 |
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