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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 16, 2024 |
| Title |
Gene regulatory divergence in spermatogenesis between mouse and opossum |
| Organisms |
Mus musculus; Monodelphis domestica |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mammalian spermatogenesis is a highly stereotyped and conserved developmental process that is essential for fitness. At the same time, gene expression in spermatogenic cells is rapidly evolving. This combination of features has been suggested to drive rapid fixation of new gene expression patterns. Using a high-resolution dataset comprising bulk and single-cell data from juvenile and adult testes of the opossum Monodelphis domestica, a model marsupial, we define the developmental timing of the spermatogenic ‘first wave’ in opossum and delineate conserved and divergent gene expression programs across the placental-marsupial split by comparison to equivalent data from mouse, a model placental mammal. Epigenomic data confirmed divergent regulation at the level of transcription, and comparison to data from four additional amniote species identified hundreds of genes with evidence of rapid fixation of expression. This gene set encompasses known and previously undescribed regulators of spermatogenic development.
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| Overall design |
scRNA-seq (10x genomics) and bulk RNA-seq from testes of mouse (Mus musculus) and opossum (Monodelphis domestica)
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| Contributor(s) |
Marshall KL, Lesch BJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 21, 2022 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Bluma J Lesch |
| Organization name |
Yale University
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| Department |
Genetics
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| Street address |
333 Cedar Street
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06525 |
| Country |
USA |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL30856 |
Illumina NovaSeq 6000 (Monodelphis domestica) |
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| Samples (26)
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| GSM6670720 |
opossum, scRNAseq, 2 |
| GSM6670721 |
opossum, 1M, 1 |
| GSM6670722 |
opossum, 1M, 2 |
| GSM6670723 |
opossum, 2M, 1 |
| GSM6670724 |
opossum, 2M, 2 |
| GSM6670725 |
opossum, 2M, 3 |
| GSM6670726 |
opossum, 2M, 4 |
| GSM6670727 |
opossum, 3M, 1 |
| GSM6670728 |
opossum, 3M, 2 |
| GSM6670729 |
opossum, 3M, 3 |
| GSM6670730 |
opossum, 3M, 4 |
| GSM6670731 |
opossum, 4M, 1 |
| GSM6670732 |
opossum, 4M, 2 |
| GSM6670733 |
opossum, 4M, 3 |
| GSM6670734 |
opossum, adult, 1 |
| GSM6670735 |
opossum, adult, 2 |
| GSM6670736 |
opossum, adult, 3 |
| GSM6670737 |
opossum, adult, 4 |
| GSM6670738 |
opossum, adult, 5 |
| GSM6670739 |
opossum, adult, 6 |
| GSM6670740 |
opossum, adult, 7 |
| GSM8289596 |
mouse_scRNAseq_3 |
| GSM8289597 |
opossum_scRNAseq_3 |
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| Relations |
| BioProject |
PRJNA893016 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216343_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of MTX, TAB, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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