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Series GSE215785 Query DataSets for GSE215785
Status Public on Mar 15, 2023
Title Transcriptomic characterization of FLC-mutant engineered human hepatocyte organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We engineered human hepatocyte organoids to study the effect of different fibrolamellar carcinoma mutations. To study the effects and differences between the different mutations, we performed bulk RNA-sequencing to understand the transcriptomic changes.
Overall design Multiple clonal lines per genotype (DNAJB1-PRKACAfus, BAP1KO, PRKAR2AKO and double BAP1KO;PRKAR2AKO) were generated and replicates of parental wild type organoids are included, all were cultured in hepatocyte medium. For wild type organoids and double mutant BAP1KO;PRKAR2AKO organoids samples were also analyzed when cultured in ductal medium.
Contributor(s) Artegiani B, Hendriks D
Citation(s) 37137901
Submission date Oct 14, 2022
Last update date Jun 01, 2023
Contact name Delilah Hendriks
Organization name Hubrecht Institute
Street address Uppsalalaan 8
City Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (31)
GSM6639969 PRKAR2A knock-out human hepatocyte organoid, clone 1
GSM6639970 PRKAR2A knock-out human hepatocyte organoid, clone 2
GSM6639971 PRKAR2A knock-out human hepatocyte organoid, clone 3
BioProject PRJNA890609

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Supplementary file Size Download File type/resource
GSE215785_processed_raw_data_FLC.csv.gz 1.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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