|
| Status |
Public on Feb 10, 2023 |
| Title |
Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep |
| Organism |
Ovis aries |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We examined how a prepubertal methionine-enriched paternal diet affected sperm DNA methylation and its subsequent effects on embryo gene expression.
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| Overall design |
Three treatment and three control rams were bred to seven ewes, and blastocysts were flushed for RNA extraction. Semen was collected from all rams and submitted for reduced representation bisulfite sequencing (RRBS) analysis.
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| |
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| Contributor(s) |
Khatib H, Townsend J, Urbano Braz C, Taylor T |
| Citation(s) |
36727109 |
| |
| Submission date |
Oct 10, 2022 |
| Last update date |
Feb 10, 2023 |
| Contact name |
Hasan Khatib |
| E-mail(s) |
hkhatib@wisc.edu
|
| Phone |
6082633484
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Animal and Dairy Sciences
|
| Street address |
1675 Observatory Dr.
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA888977 |
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