|
| Status |
Public on Jan 31, 2023 |
| Title |
Dense, systematic mutagenesis to identify the features required for CSEs |
| Organisms |
Mus musculus; synthetic construct |
| Experiment type |
Other
|
| Summary |
Investigate the sequence features required for CSE activity and selectivity.
|
| |
|
| Overall design |
Candidate region was synthesized as 80 190 nt oligonucleotides tiled in 5 nt steps. Each wild type 190 nt oligonucleotide was mirrored by a matching mutant in which the central 5 nt were deleted. The activity of mutant and mirrored wt oligos was compared to identify the import sequence.
|
| |
|
| Contributor(s) |
Cao Y, Zhang X, Akerberg BN, Pu WT |
| Citation(s) |
36705030 |
| |
| Submission date |
Oct 07, 2022 |
| Last update date |
May 02, 2023 |
| Contact name |
Xiaoran Zhang |
| E-mail(s) |
xiaoran.zhang@childrens.harvard.edu
|
| Phone |
8574259855
|
| Organization name |
Boston Children's Hospital
|
| Department |
Cardiology
|
| Lab |
Pu lab
|
| Street address |
14 Perkins Square, Apt 11
|
| City |
Jamaica Plain |
| State/province |
MA |
| ZIP/Postal code |
02130 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL26526 |
Illumina NovaSeq 6000 (synthetic construct) |
|
| Samples (14)
|
|
| This SubSeries is part of SuperSeries: |
| GSE215065 |
Regulation of mouse chamber selective enhancers |
|
| Relations |
| BioProject |
PRJNA888108 |
Less...
More...