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| Status |
Public on Apr 30, 2011 |
| Title |
Transcriptomic analysis of targets regulated by miR-125b in primary endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human vein umbilical endothelial cells (HUVEC) were transfected with pre-miR control and pre-miR 125b (Ambion) in order to identify targets (direct and indirect) downregulated by miR-125b in endothelial cells. 317 transcripts were downregulated with a fold change ≥ 1.5, among which 99 are predicted direct targets of mir-125b. Computional approach performed with Ingenuity Pathway Knowledge shows that movement of cell and cell-to-cell signalling are the top two biological functions affected bewteen control and transfected cells. Experiments performed in zebrafish and in HUVEC confirmed that mir-125b mainly affects genes involved in cellular movements and junctions.
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| Overall design |
Total RNA was obtained from cells transfected in HUVEC with pre-miR control and pre-miR 125b. Three independent transfections were performed for each condition.
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| Contributor(s) |
Malvaux L, Pendeville H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 22, 2010 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Ingrid Struman |
| E-mail(s) |
i.struman@ulg.ac.be
|
| Organization name |
GIGA-R
|
| Department |
BMGG
|
| Street address |
Avenue de l'Hôpital, 1
|
| City |
Liege |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA125811 |
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