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Series GSE21413 Query DataSets for GSE21413
Status Public on Aug 01, 2010
Title Expression profile of proliferating and differentiating primary human keratinocytes in vitro
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human epidermal keratinocytes undergo tightly controlled program of cell differentiation, leading to the formation of cornified envelope. Primary keratinocytes in vitro, under calcium stimulation mimic the differentiation program observed in vivo. Analysis of the transcription profile of two cell population, such as proliferating cells and differentiating cells helps to discover new genes implicated in that process and to understand the mechanisms of regulation of the keratinocyte differentiation.
Overall design Primary human keratinocytes were cultured under proliferating (Day 0, sub-confluent cells) and differentiating (seven days of high calcium medium) conditions. As a source of cells, we used normal skin from different body sites: back, foreskin, palmoplantar. RNA extracted from cultured primary human keratinocytes were isolated from five different donors. We compared the expression profiles of proliferating versus differentiating keratinocytes.
Contributor(s) Obarzanek-Fojt M, Favre B, Huber M, Hohl D, Harshmann K, Pradervand S, Hagenb├╝chle O, Paillusson A
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Submission date Apr 20, 2010
Last update date Dec 06, 2018
Contact name Magdalena Obarzanek-Fojt
Phone (41) 21 314 03 64
Fax (41) 21 314 03 78
Organization name CHUV
Department Dermatology
Lab Cutanous Biology
Street address Beaumont 29
City Lausanne
ZIP/Postal code 1011
Country Switzerland
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (10)
GSM535476 keratinocytes_proliferating_rep1
GSM535477 keratinocytes_differentiating_rep1
GSM535478 keratinocytes_proliferating_rep2
BioProject PRJNA126355

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Supplementary file Size Download File type/resource
GSE21413_RAW.tar 31.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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