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| Status |
Public on Sep 30, 2022 |
| Title |
Soluble CD83 improves and accelerates wound healing by the induction of pro-resolving macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To facilitate the recovery process of chronic and hard-to-heal wounds novel pro-resolving treatment options are urgently needed. We investigated the pro regenerative properties of soluble CD83 (sCD83) on cutaneous wound healing, where sCD83 accelerated wound healing not only after systemic but also after topical application, which is of high therapeutic interest. Cytokine profile analyses revealed an initial upregulation of inflammatory mediators such as TNFa and IL-1b, followed by a switch towards pro-resolving factors, including YM-1 and IL-10, both expressed by tissue repair macrophages. These cells are known to mediate resolution of inflammation and stimulate wound healing processes by secretion of growth factors such as epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF), which promote vascularization as well as fibroblast and keratinocyte differentiation. In conclusion, we have found strong wound healing capacities of sCD83 beyond the previously described role in transplantation and autoimmunity. This makes sCD83 a promising candidate for the treatment of chronic- and hard-to-heal wounds
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| |
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| Overall design |
RNA-seq analysis of biopsies derived from sCD83 (100µg)- or mock-treated C57BL/6 mice 3 days after wound infliction via 6mm biopsy skin punches
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| |
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| Contributor(s) |
Royzman D, Fuchs M |
| Citation(s) |
36248909 |
| |
| Submission date |
Sep 23, 2022 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Maximilian Fuchs |
| E-mail(s) |
maximilian.fuchs@item.fraunhofer.de
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| Organization name |
Fraunhofer Institute for Toxicology and Experimental Medicine ITEM
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| Department |
Cardiovascular Research
|
| Street address |
Nikolai-Fuchs-Str. 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA883724 |
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