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| Status |
Public on Sep 13, 2023 |
| Title |
Il-6 signaling exacerbates hallmarks of tendon lesions by stimulating progenitor proliferation & migration to damage. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We leveraged an explant-based, tissue engineered model of tendon damage to study cross-compartmental interactions between the tendon core and extrinsic progenitors, as well as between the tendon core and extrinsic macrophages. We suspected IL-6 signaling to play a major role in this crosstalk, so we also incorporated core explants from IL-6 knock-out mice.
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| Overall design |
1) Comparative gene expression profiling analysis of RNA-seq data for wildtype core explants or IL-6 knock-out core explants surrounded by a cell-free hydrogel, one filled with extrinsic progenitors, or one filled with macrophages. 2) Comparative gene expression profiling analysis of RNA-seq data for extrinsic progenitors surrounding a wildtype or a knock-out core.
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| Web link |
https://doi.org/10.7554/eLife.87092.1
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| Contributor(s) |
Stauber T, Moschini G, Hussien AA, Jaeger PK, De Bock K, Snedeker JG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 23, 2022 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Tino Stauber |
| E-mail(s) |
staubert42@gmail.com
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| Organization name |
ETH Zurich
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| Street address |
Lengghalde 5
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| City |
Zurich |
| ZIP/Postal code |
8008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (32)
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| GSM6597870 |
3-150-1_Core_N2: core, wildtype core // cell-free, rep 1 |
| GSM6597871 |
3-150-2_Core_N2: core, wildtype core // progenitors, rep 1 |
| GSM6597872 |
3-150-2_Prog_N1: progenitors, wildtype core // progenitors, rep 1 |
| GSM6597873 |
3-150-2_Prog_N2: progenitors, wildtype core // progenitors, rep 2 |
| GSM6597874 |
3-150-3_Core_N2: core, wildtype core // macrophages, rep 1 |
| GSM6597875 |
3-150-4_Core_N2: core, IL-6 knock-out core // cell-free, rep 1 |
| GSM6597876 |
3-150-5_Core_N2: core, IL-6 knock-out core // progenitors, rep 1 |
| GSM6597877 |
3-150-5_Prog_N1: progenitors, IL-6 knock-out core // progenitors, rep 1 |
| GSM6597878 |
3-150-5_Prog_N2: progenitors, IL-6 knock-out core // progenitors, rep 2 |
| GSM6597879 |
3-152-1_Core_N1: core, wildtype core // cell-free, rep 2 |
| GSM6597880 |
3-152-1_Core_N2: core, wildtype core // cell-free, rep 3 |
| GSM6597881 |
3-152-2_Core_N1: core, wildtype core // progenitors, rep 2 |
| GSM6597882 |
3-152-2_Core_N2: core, wildtype core // progenitors, rep 3 |
| GSM6597883 |
3-152-2_Prog_N1: progenitors, wildtype core // progenitors, rep 3 |
| GSM6597884 |
3-152-2_Prog_N2: progenitors, wildtype core // progenitors, rep 4 |
| GSM6597885 |
3-152-3_Core_N1: core, wildtype core // macrophages, rep 2 |
| GSM6597886 |
3-152-3_Core_N2: core, wildtype core // macrophages, rep 3 |
| GSM6597887 |
3-152-4_Core_N1: core, IL-6 knock-out core // cell-free, rep 2 |
| GSM6597888 |
3-152-4_Core_N2: core, IL-6 knock-out core // cell-free, rep 3 |
| GSM6597889 |
3-152-5_Core_N1: core, IL-6 knock-out core // progenitors, rep 2 |
| GSM6597890 |
3-152-5_Core_N2: core, IL-6 knock-out core // progenitors, rep 3 |
| GSM6597891 |
3-152-5_Prog_N1: progenitors, IL-6 knock-out core // progenitors, rep 3 |
| GSM6597892 |
3-152-5_Prog_N2: progenitors, IL-6 knock-out core // progenitors, rep 4 |
| GSM6597893 |
3-154-1_Core_N2: core, wildtype core // cell-free, rep 4 |
| GSM6597894 |
3-154-2_Core_N2: core, wildtype core // progenitors, rep 4 |
| GSM6597895 |
3-154-2_Prog_N1: progenitors, wildtype core // progenitors, rep 5 |
| GSM6597896 |
3-154-2_Prog_N2: progenitors, wildtype core // progenitors, rep 6 |
| GSM6597897 |
3-154-3_Core_N2: core, wildtype core // macrophages, rep 4 |
| GSM6597898 |
3-154-4_Core_N2: core, IL-6 knock-out core // cell-free, rep 4 |
| GSM6597899 |
3-154-5_Core_N2: core, IL-6 knock-out core // progenitors, rep 4 |
| GSM6597900 |
3-154-5_Prog_N1: progenitors, IL-6 knock-out core // progenitors, rep 5 |
| GSM6597901 |
3-154-5_Prog_N2: progenitors, IL-6 knock-out core // progenitors, rep 6 |
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| Relations |
| BioProject |
PRJNA883640 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214015_DEG_KOCore-over-WTCore_Progs.xlsx |
14.8 Mb |
(ftp)(http) |
XLSX |
| GSE214015_DEG_KOCorecellfree-over-WTCorecellfree_Core.xlsx |
14.5 Mb |
(ftp)(http) |
XLSX |
| GSE214015_DEG_KOCoreprogs-over-WTCoreprogs_Core.xlsx |
12.8 Mb |
(ftp)(http) |
XLSX |
| GSE214015_DEG_WTCoremacros-over-WTCorecellfree_Core.xlsx |
13.0 Mb |
(ftp)(http) |
XLSX |
| GSE214015_DEG_WTCoreprogs-over-WTCorecellfree_Core.xlsx |
12.9 Mb |
(ftp)(http) |
XLSX |
| GSE214015_RAW.tar |
4.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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