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Series GSE214014 Query DataSets for GSE214014
Status Public on Jan 01, 2024
Title Krüppel-like factor 2 in VCAM1+ endothelial cells is a critical regulator of vascular inflammation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Endothelial cells (ECs) act as an internal barrier to the vessel wall, hindering the development of inflammation, and only when activated will promote the development of vascular inflammation. However, the heterogeneity of the aortic ECs in the setting of vascular inflammation have not been fully assessed. Here, we defined 4 EC subpopulations, including Cd36+ EC, Notch3+ EC, Vcam1+ EC and Lyve1+ EC. Based on the enrichment analysis, Vcam1+ EC were considered to be a subpopulation of ECs susceptible to activation. After high-fat diet (HFD) intake, decreased expression of Krüppel-like factor 2 (Klf2) occurred only in Vcam1+ EC. Moreover, S1pr1 was validated as a downstream target protein of klf2. Upregulation of Klf2 expression ameliorated vascular ECs inflammation under HFD condition. Our study elucidates the Klf2 in Vcam1+ EC is a critical regulator of vascular inflammation and increases understanding of development and progression of aortic inflammatory disease.
Overall design Single cell RNA-seq on aortic cells of normal diet or high-fat diet mice
Contributor(s) Kan H, Zhang K, Mao A
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Submission date Sep 23, 2022
Last update date Jan 01, 2024
Contact name Hao Kan
Phone +8617714542580
Organization name jiangnan university
Street address The number of 1800 ,Li Hu Road
City Wuxi
State/province -
ZIP/Postal code 214122
Country China
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM6597864 Control, replicate 1, scRNAseq
GSM6597865 Control, replicate 2, scRNAseq
GSM6597866 Control, replicate 3, scRNAseq
BioProject PRJNA883634

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE214014_RAW.tar 332.4 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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