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Series GSE213600 Query DataSets for GSE213600
Status Public on Jan 11, 2023
Title PPARg and C/EBPa response to acute cold stress in brown adipose tissue [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Brown adipose tissue (BAT) has the ability to burn calories as heat. BAT thermogenesis is an attractive way to combat obesity pandemic. However, the transcriptional network resulting in lipid synthesis to oxidation shift during thermogenesis is not completely understood. Here, we report the regulation of two master regulators of adipogenesis, PPARγ and C/EBPα, during acute cold stress in BAT. We found PPARγ dissociated from DNA in a third of its binding sites and these included cebpa enhancers leading to decreased C/EBPα. This dissociation required PPARγ binding to activating ligands, thus modulated by diet. Meanwhile PPARα also detached from DNA, and co-activator PGC1α associated with ERR1 as part of transcriptional network regulating lipid metabolism. Subsequent global replacement of C/EBPα by C/EBPβ and its associated transcriptional machinery was required for upregulation of structural lipid synthesis despite general upregulation of fatty acid oxidation.
 
Overall design Various ChIPseq of BAT
 
Contributor(s) Lau KH, Waldhart AN, Dykstra H, Avequin T, Wu N
Citation(s) 36624847
Submission date Sep 18, 2022
Last update date Jan 12, 2023
Contact name Ning Wu
E-mail(s) ning@omnispacemail.com
Organization name Van Andel Institute
Street address 333 Bostwick Ave NE
City Grand Rapids
State/province MI
ZIP/Postal code 49503
Country USA
 
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (52)
GSM6589616 BAT PPARG ChIPseq at RT, rep 1
GSM6589617 BAT PPARG ChIPseq at RT, rep 2
GSM6589618 BAT PPARG ChIPseq at RT, rep 3
This SubSeries is part of SuperSeries:
GSE213601 PPARg and C/EBPa response to acute cold stress in brown adipose tissue
Relations
BioProject PRJNA881931

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE213600_RAW.tar 11.5 Gb (http)(custom) TAR (of BW, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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