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Status |
Public on Dec 31, 2022 |
Title |
Whole-organism C. elegans L2 GRH-1 ChIP-seq (experiment 1 of 2) |
Organism |
Caenorhabditis elegans |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We performed ChIP-seq to determine chromatin regions binding Transcription Factor GRH-1, and thereby potential binding motifs and sites thereof, in C. elegans (larval stage 2) whole organisms.
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Overall design |
One sample was used as "input", ie non-specific pulldown. 3 GRH-1-specific samples were generated, using 3 different salt concentrations for protocol optimisation and potential replication. Samples were collected at two time points to broadly cover the L2 stage, before pooling.
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Contributor(s) |
Azzi C, Braun K, Smith AA, Gaidatzis D, Meeuse MW, Grosshans H |
Citation(s) |
36688410 |
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Submission date |
Sep 16, 2022 |
Last update date |
Apr 03, 2023 |
Contact name |
Helge Grosshans |
E-mail(s) |
helge.grosshans@fmi.ch
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Organization name |
Friedrich Miescher Institute for Biomedical Research
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Department |
Multicellular Systems
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Lab |
Biological clocks and timers in development
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Street address |
Maulbeerstrasse 66
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City |
Basel |
State/province |
Basel-Stadt |
ZIP/Postal code |
4058 |
Country |
Switzerland |
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Platforms (1) |
GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE213510 |
Whole-organism C. elegans L2 GRH-1 ChIP-seq |
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Relations |
BioProject |
PRJNA881277 |