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| Status |
Public on Jan 04, 2023 |
| Title |
Areas of H3K27 tri-methylation in CD8+ T cells from the spleens of LCMV-Armstrong or LCMV-Clone 13 infected mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The profiles of H3K27 tri-methylation in CD8+ T cells from LCMV-Armstrong and LCMV-Clone 13 infected mice are known to be distinct from one another. We used CUT&RUN (Cleavage Under Targets and Release Using Nuclease) to analyze these differences in splenic CD8+ T cells of these two infection conditions.
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| Overall design |
Adoptively transferred CD8+ P14 T cells from the spleen of C57BL/6 mice were isolated by Fluorescence-activated cell sorting (FACS) at day 7 post-infection according to the presence of CD45.1 congenic marker signal and analyzed using CUT&RUN.
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| Contributor(s) |
Quezada L, Jin W |
| Citation(s) |
36716323 |
| |
| Submission date |
Sep 15, 2022 |
| Last update date |
Apr 05, 2023 |
| Contact name |
Gene W Yeo |
| E-mail(s) |
vincenzojin@gmail.com
|
| Phone |
+1-650-695-9262
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| Organization name |
University of California, San Diego
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| Department |
Cellular and Molecular Medicine
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| Street address |
9500 Gilman Dr
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| City |
La Jolla |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA880890 |
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