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| Status |
Public on Sep 29, 2023 |
| Title |
Phase-separated CCER1 coordinates histone-to-protamine transition and fertility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Idiopathic fertility disorders are associated with mutations in various genes. Here, we report that coiled-coil glutamate-rich protein 1 (CCER1), a germline-specific and intrinsically disordered protein (IDP), mediates post-meiotic sperm differentiation, while CCER1 deficiency results in defective sperm chromatin compaction and infertility in mice. CCER1 increases transition protein (Tnp1/2) and protamine (Prm1/2) transcription and serves as a multifaceted mediator for multiple epigenetic modification events of histones during the histone to protamine transition (HTP). Immiscible with heterochromatin in the nucleus, CCER1 self-assembles into polymer droplet and acts as a liquid-liquid phase separated condensate in the nucleus. Notably, we identified the loss-of function variants of human CCER1 (hCCER1) in five patients with non-obstuctive azoospermia (NOA) while absent in 2,713 fertile controls. The mutants led to premature termination or frameshift of CCER1 translation and disrupted condensates in vitro. In conclusion, we propose that nuclear CCER1 is a novel phase-separated condensate that links histone epigenetic modifications, sperm HTP transitions, chromatin condensation, and male fertility.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for Ccer1 WT and KO testis.
To confirm the condensate state in the Ccer1 -/- mutants, we took advantage of in situ Hi-C to illustrate the chromatin state in both Ccer1 +/+ and Ccer1-/- sperm at the molecular level."
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| Contributor(s) |
Qin D, Zhang Y, Gu Y, Wang S, Xie W, Hu Z, Wu X |
| Citation(s) |
38081819 |
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| Submission date |
Sep 06, 2022 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Jimiao Gao |
| E-mail(s) |
Gaojimiao_Gloria@stu.njmu.edu.cn
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| Phone |
+8618061621262
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| Organization name |
Nanjing Medical University
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| Street address |
101Longmian Avenue, Jiangning District, Nanjing 211166, P.R. China
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| City |
Nanjing |
| ZIP/Postal code |
211166 |
| Country |
China |
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| Platforms (2) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA877103 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212733_RAW.tar |
2.7 Gb |
(http)(custom) |
TAR (of HIC, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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