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Series GSE21209 Query DataSets for GSE21209
Status Public on Mar 25, 2011
Title Gene expression changes upon knockdown or over-expression of the ETS gene ETV4 in prostate cancer cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chromosomal abnormalities that give rise to elevated expression levels of the ETS genes ETV1, ETV4, ETV5, or ERG are prevalent in prostate cancer, but the function of these transcription factors in carcinogenesis is not clear. Previous work implicates ERG, ETV1, and ETV5 as regulators of invasive growth but not transformation in cell lines. Here we show that the PC3 prostate cancer cell line provides a model system to study the over-expression of ETV4. Anchorage independent growth assays and microarray analysis indicate that high ETV4 expression is critical for the transformation phenotype of PC3 cells. However, genes up-regulated upon ETV4 over-expression were very similar to genes up-regulated by ETV1 over-expression in the RWPE-1 normal prostate cell line. Together these data indicate that the ETV4 dependent transformation phenotype observed in PC3 cells is due to the genetic background of the cell line, rather than a distinct characteristic of ETV4. Furthermore, these findings suggest that the function of ETS genes in prostate cancer may differ based on other genetic alterations in a tumor.
Overall design Two sets of two color experiments. First is PC3 cells expressing one of two independent ETV4 shRNAs versus PC3 cells expressing a control shRNA (luciferase). Second is RWPE-1 cells expressing 3xFlag tagged ETV4 versus RWPE-1 cells with a control (empty) vector.
Contributor(s) Hollenhorst PC, Graves BJ, Paul L
Citation(s) 21373373
Submission date Apr 05, 2010
Last update date Feb 22, 2018
Contact name Peter Hollenhorst
Organization name Indiana University
Street address 1001 E Third St, Jordan Hall 104
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (7)
GSM530332 Knockdown of ETV4 in PC3 cells using first shRNA (ETV4a) replicate 1
GSM530333 Knockdown of ETV4 in PC3 cells using first shRNA (ETV4a) replicate 2
GSM530334 Knockdown of ETV4 in PC3 cells using second shRNA (ETV4b) replicate 1
BioProject PRJNA126299

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Supplementary file Size Download File type/resource
GSE21209_RAW.tar 110.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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