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| Status |
Public on Sep 15, 2022 |
| Title |
Long-term engraftment of engineered human plasma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Due to their unique longevity and capacity to secrete high levels of protein, plasma B cells play have the potential to be used as a cell therapy for protein replacement. Here, we show that ex vivo engineered human plasma cells exhibited transcriptional features of long-lived plasma cells.
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| Overall design |
Ex vivo culture human B cell with proliferation and differentiation stimulants. Followed by CD138 isolation and CD38 isolation at day13 (D11), collecting isolated cell and keep culture this two groups (CD138+ vs CD38+) of cells. After 6 days, harvest these two groups of cells at day19(D17). All the collected cells are put in trizol and store in -20.
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| Web link |
https://www.nature.com/articles/s41467-022-33787-8
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| |
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| Contributor(s) |
Cheng RY, James R |
| Citation(s) |
36245034 |
| Submission date |
Aug 24, 2022 |
| Last update date |
Jan 26, 2023 |
| Contact name |
Rene Cheng |
| E-mail(s) |
rene271828@gmail.com
|
| Organization name |
Seattle children research institute
|
| Street address |
1900 9th AVE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA873089 |
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