|
| Status |
Public on Sep 16, 2022 |
| Title |
Probing cell identity hierarchies by fate titration and collision during direct reprogramming [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Collide-seq: A systematic comparison of how different reprogramming transcription factors achieve conversion as well as an in-depth analysis of how cells resolve the conflict when more than one fate is instructed.
|
| |
|
| Overall design |
Mouse embryonic fibroblasts were nucleofected with doxycycline inducible constructs for the indicated reprogramming transcription factors. Cells were expanded for about 10 days and positive cells sorted using FACS. All experimental conditions were pooled and transcription factor expression induced for the indicated time points before scRNA-seq.
|
| Web link |
https://www.embopress.org/doi/full/10.15252/msb.202211129
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| |
|
| Contributor(s) |
Hersbach BA, Fischer DS, Masserdotti G, Deeksha D, Mojžišová K, Waltzhöni T, Rodriques-Terrones D, Heinig M, Theis FJ, Götz M, Stricker SH |
| Citation(s) |
36106915 |
| |
| Submission date |
Aug 23, 2022 |
| Last update date |
Sep 18, 2022 |
| Contact name |
Bob Hersbach |
| E-mail(s) |
bob.hersbach@helmholtz-muenchen.de
|
| Organization name |
Helmholtz Zentrum Munich
|
| Department |
Institute of Stem Cell Research
|
| Street address |
Großhaderner Str. 9
|
| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
| GSM6504517 |
Ascl1-Hnf1a-Myod1-Oct4, 48h biol rep 2 |
| GSM6504518 |
Ascl1-Foxa2-Myod1-Oct4-Sox2 72h tech rep 1 |
| GSM6504519 |
Ascl1-Foxa2-Myod1-Oct4-Sox2 72h tech rep 2 |
|
| This SubSeries is part of SuperSeries: |
| GSE211864 |
Probing cell identity hierarchies by fate titration and collision during direct reprogramming |
|
| Relations |
| BioProject |
PRJNA872403 |
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