Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
Summary
To investigate the change of embryo transcriptome upon the inhibition of transition, we treated the late zygote with CHX and performed RNA-seq; To test KLF17 necessity for ZGA transcripts expression and embryo development, we next generated conditional knockout mouse models for Klf17 and collected embryo to perform RNA-seq and Stacc-seq.
Overall design
We selected seven stages of oocyte and embryo when we treated embyo with CHX; For the transcriptome of maternal Klf17 knockout embryo, We selected two stages of embryo; For the Stacc-seq of maternal Klf17 knockout embryo, only early 2 cell stage of embryo was used.
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